Figure 2

Determination of hMSC effect in alloreactivity. (A) Differences in survival, (B) GVHD clinical score, and (C) donor T cell expansion in the 3 groups of mice. Lethally irradiated BALB/c host mice were given intravenous injections of 2 × 10^6 TCDBM cells from C57BL/6 donors with or without 0.25 × 10 ^6 CD4+ T cells from donors and 1x 10 ^6 hMSCs. Multiple doses of hMSCs at the day of transplantation then 3d and 6d after transplantation were given for observation of survival and GVHD score. A single dose of hMSCs was given at the day of transplantation for testing of donor T cell expansion. (A) Survival of irradiated hosts given TCDBM cells alone (n = 10) or with 0.25 × 10^6 CD4+ T cells (n = 20) or with 0.25 × 10^6 CD4+ T cells and 1 × 10^6 hMSCs (n = 12) (p = 0.0035). (B) GVHD score of host mice and (C) relative and absolute numbers of donor CD3 T cells in BALB/c host tissue 6d after transplantation with TCDBM alone or with CD4+ T cells , or CD4+ T cells and hMSCs. (*p <0.05). Data were combined from a single experiment with 8 mice per group for the GVHD scoring and 4 mice per group in the experiment for determination of donor T cell engraftment.