Figure 3

Effects of combination therapy with valproate and prednisolone in SU-DHL-8 cells. SU-DHL-8 cells were incubated with or without 1 mM valproate and/or 55 μM prednisolone in cell culture media. After 48 hours, cells were harvested and levels of CD20 mRNA were estimated by qPCR (A). Quantification of anti-CD20 antibodies bound to the cell surface was estimated using FACS and QuantiBRITE assay (B). Mean values are from five separate experiments, bars represent standard deviation.