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Figure 3 | Experimental Hematology & Oncology

Figure 3

From: Lymphocytes from chronic lymphocytic leukaemia undergo ABL1-linked amoeboid motility and homotypic interaction as an early adaptive change to ex vivo culture

Figure 3

Detailed analysis of the shape forms and cytoskeletal rearrangement observed for the CLL lymphocytes in culture. Panels A & B. Confocal microscopy showing F-actin cytoskeletal arrangement of CLL lymphocytes during motility (A) and conventional fluorescent microscopy (B) (TR-phalloidin probed for F-actin at z planes, Dapi nuclear stain as indicated). F-actin is enriched within extended projections, but no clear lamellipodium or posterior attachment is formed. Panel C. Representative histogram of shape change (measured as aspect ratio intensity a weighted measure of shape and F-actin polarisation in which high levels indicate round non-polar cells) revealing that there are two shape-form populations at fixed time points within the culture (confirmed by 4 separate assessments of adherent or aspirated cells from culture). Panel D. A similar dual population of F-actin content is indicated by flow cytometric analysis of the cultured cells (Flow cytometric analysis using NBD-phallacidin probe).

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