Figure 3From: Lymphocytes from chronic lymphocytic leukaemia undergo ABL1-linked amoeboid motility and homotypic interaction as an early adaptive change to ex vivo culture Detailed analysis of the shape forms and cytoskeletal rearrangement observed for the CLL lymphocytes in culture. Panels A & B. Confocal microscopy showing F-actin cytoskeletal arrangement of CLL lymphocytes during motility (A) and conventional fluorescent microscopy (B) (TR-phalloidin probed for F-actin at z planes, Dapi nuclear stain as indicated). F-actin is enriched within extended projections, but no clear lamellipodium or posterior attachment is formed. Panel C. Representative histogram of shape change (measured as aspect ratio intensity a weighted measure of shape and F-actin polarisation in which high levels indicate round non-polar cells) revealing that there are two shape-form populations at fixed time points within the culture (confirmed by 4 separate assessments of adherent or aspirated cells from culture). Panel D. A similar dual population of F-actin content is indicated by flow cytometric analysis of the cultured cells (Flow cytometric analysis using NBD-phallacidin probe).Back to article page