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Figure 1 | Experimental Hematology & Oncology

Figure 1

From: Phenethyl isothiocyanate and paclitaxel synergistically enhanced apoptosis and alpha-tubulin hyperacetylation in breast cancer cells

Figure 1

Synergistic effect of phenethyl isothiocyanate (PEITC) and paclitaxel (taxol) on acetylation of alpha-tubulin and expression of tubulins in breast cancer cells. The MCF7 cells were treated with 5 μM of PEITC and 10 nM of taxol alone or in combination. Due to higher resistance of MDA-MB-231 cells, PEITC at 10 μM and taxol at 100 nM were used. Proteins were immunoprecipitated with appropriate antibodies and were detected by Western blotting as described in Materials and methods. β-actin level was used as an internal loading control for protein amount. The relative expression level over control cells was calculated after scanning of the density of each protein band with a densitometer. A representative blot from three or more independent experiments was shown.

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