Figure 5

ELISA quantification of IL-8 and TNFα in THP-1 cells. Non-activated or LPS-activated THP-1 cells were incubated in defibrinated plasma in the presence of 0.35 μg/ml rivaroxaban or 1.5 μg/ml fondaparinux. After 24 hours, IL-8 in the supernatants was measured by ELISA. The data present is means ± SEM of 3 independent experiments. A: ELISA for IL-8. Statistic significance (p < 0.05) was found for the differences between non-activated cells versus control plasma; between non-activated versus LPS-activated cells; and between the activated cells treated by fondaparinux versus LPS-activated cells alone. B: ELISA for TNFα. Statistic significance (p < 0.05) was found for the differences between activated cells versus the control plasma; between activated cells treated by fondaparinux versus LPS-activated cells alone.