Figure 3
Thrombin generation and AT III activity. (A) Thrombin generation in platelet-poor plasma induced by the addition of LPS-activited THP-1. Time–dependent thrombin generation was evaluated using a synthetic substrate SQ 150. Activated THP-1 cells were washed and added to 240 μl of defibrinated plasma and CaCl2. Fondaprinux at 1.5 μg/ml or 0.35 μg/ml rivaroxaban were added into the test in order to evaluate their inhibitory effect. Optical density was measured. Significant differences were found among the three samples after 5 min reaction. (B) Antithrombin III activity in the absence or presence of rivaroxaban and fondaparinux in platelet-poor plasma (PPP) mixed with non-activated or PLS-activated THP-1 cells. The results are expressed as the percentage of AT activity in PPP. No statistical difference was found.