Effect of the type and means of chemotherapy administration on Tregs. (A) Differential distribution of Treg/Teff ratio in the peripheral blood of patients treated with anti-mitotic, anti-DNA or combined (anti-mitotic/anti-DNA) regimens in a metronomic or standard pattern. Shown are means ± SE. All samples were analyzed in duplicates. (B) Teff proliferation expressed as mean OD at 450 nm ± SE. All samples were analyzed in triplicates. Other details as in Figure 2. * p < 0.05, ** p < 0.01, as estimated by Mann–Whitney or paired-t test.