Figure 4
From: Silence of MCL-1 upstream signaling by shRNA abrogates multiple myeloma growth
Effect of STAT3, PI3K and MAPK gene silencing on MM cell apoptosis and cell growth. Lentirirus-transfected human U266 and 8226 MM cells (bearing shSTAT3, shPI3K, shMAPK or control shRNA) were cultured in RPMI-1640 medium in presence of IL-6 for 48 hours. (A and B) The cells were harvested and subjected to apoptotic analyses. The percentage of apoptotic cells in U266 or 8226 cells was presented. (C and D) The cell growth was measured by MTT assay and presented as absorbance values at 570 nm. Data were represented from three independent experiments. *, P < 0.05; **, P < 0.01.