Figure 3
From: Silence of MCL-1 upstream signaling by shRNA abrogates multiple myeloma growth
Interaction among STAT3, PI3K and MAPK. Total protein was isolated from IL-6 treated human 8226 cells transfected with lentivirus bearing shSTAT3 (A), shPI3K (B), shMAPK1 (C), shMAPK2 (D), or combined shSTAT3 + shPI3K + shMAPK2 (E), and shRNA control. The proteins were profiled by Western blot with specific antibodies against STAT3, pSTAT3, PI3K, MAPK, pMAPK, MCL-1 or β-actin. Data represented one of two independent experiments.