Figure 1
From: Silence of MCL-1 upstream signaling by shRNA abrogates multiple myeloma growth
IL-6 enhanced MCL-1 expression and MM cell growth. Human U266 and 8226 MM cells were cultured in RPMI and stimulated with IL-6 for 0, 24 or 48 hours. (A) MCL-1 gene expression in the cells was quantified by qRT-PCR. mRNA fold change was presented. (B) MCL-1 protein expression was detected by Western blot with anti-MCL-1 specific antibodies. (C) MCL-1 protein expression in IL-6 treated group was quantified based on β-actin level and in comparison with untreated cells. (D and E) U266 and 8226 MM cell growth after IL-6 treatment was measured by MTT assay. Cell growth was presented as OD values. Data represents three independent experiments. *, P < 0.05; **, P < 0.01.