Figure 6

Effect of TAK-475 on CD-20 expression and chemoimmuno-sensitivity. MEC-2 cells were treated with 10 μM TAK-475 (T) for 3 days, processed for double immunostaining, and photographed by confocal microscope (A-D). The results represent three experiments performed with duplicate samples. The cells were also treated with 10 μM fludarabine (F), 10 μg/ml rituximab (R) or their combinations (F + R) in the presence or absence of 10 μM TAK-475 for 3 days, and then analyzed cell viability by MTT assay (E). The values of treated with TAK-475 samples (n = 16) were statistically different from the controls (C). *P < 0.05. **P < 0.01.