Figure 4
The influence of BIBB-515 and YM-53601 on cholesterol levels and CD20 expression in MEC-2 cells. MEC-2 cells were treated with 10 μM either BIBB-515 (B) or YM-53601 (Y) for 3 days. A) The cells were extracted and analyzed for total cellular cholesterol. The values of treated with BIBB-515 or YM-53601 samples were statistically different from the control (C, without BIBB-515 or YM-53601) (n = 5). *P < 0.05. B-G) The cells were stained for 1 hour with anti-CD-20 APC antibody and cholera toxin-B FITC, and observed and photographed by confocal microscope. The results represent three experiments performed with duplicate samples.