Figure 2

Quantitative expression of NGF, CNTF, and BDNF mRNAs in cells of the BMMC fraction obtained from three healthy donors. 20 ng cDNA was used as PCR template. Real-time PCR mixture was prepared with KAPA SYBR® FAST qPCR master mix. β-actin was used as the reference gene. Each bar represents mean ± SD from triplicate of each sample. The expression levels were scaled relative to the lowest unscaled expression level for the same gene as the sample of interest.