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Figure 1 | Experimental Hematology & Oncology

Figure 1

From: Genetic profiles of plasmacytoid (BDCA-4 expressing) DC subtypes-clues to DC subtype function in vivo

Figure 1

Separation of BDCA-4 subpopulation by MACS and FACS. Representative Flow Cytometry plot evaluating the purity of BDCA-4 populations from Ficol-separated PBMCs obtained from normal healthy donors. Leukocytes stained with BDCA-4-PE and the lymphocyte markers CD3-FITC and CD20-FITC and evaluated by flow cytometry: Prior to sorting (A), after Magnetic Bead Sorting (MACS) (B), and after MACS followed by Fluorescence-Activated Cell Sorting (FACS) (C). R2 indicates the left upper quadrant sorting gate, R3 indicates gate for lymphocytes removed by FACS.

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Experimental Hematology & Oncology

ISSN: 2162-3619

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