Kinetics of BCR-induced expressions of EGR-1 and c-MYC in MCL cell lines and primary MCL cells. (A) Kinetics of BCR-induced mRNA expression of EGR-1 and c-MYC in Granta-519 and HBL-2 cell lines. 3×106 cells/ml were stimulated with 10 μg/ml of immobilized anti-IgM antibody for 15 min, 30 min, 1 h, 3 h and 6 h. EGR-1 and c-MYC expressions were analyzed by qRT-PCR. (B) Induction of EGR-1 protein upon BCR engagement was confirmed by western-blot. (C) The same experiments were performed on primary patients’ cells. EGR-1 and c-MYC expressions were analyzed by qRT-PCR (left panel) and western-blot (right panels). (D) EGR-1 and c-MYC mRNA expressions upon anti-IgM stimulation (1 h) were analyzed by qRT-PCR from 7 patients’ samples (UPN2, 3, 4, 5, 6, 7 and 8). Fold increase of mRNA level were calculated compared with unstimulated cells in all experiments. All measurements were done in duplicate and the mean is provided.