Clinical and laboratory characteristics of adolescents with platelet function disorders and heavy menstrual bleeding

Table 3 Abnormal platelet testing and electron microscopy studies in adolescents with platelet function disorders

	Prolonged Closure Times ^*	Impaired Platelet Aggregation Responsiveness by Agonist ^*					EM Reduced DG/PL ^*
Patient Group	C-EPI, C-ADP	EPI	ADP	Ristocetin	AA	Collagen	<3.68 DG/PL
	(n=43)			(n=35^§)			(n=28)
Heavy Menstrual Bleeding	18 (41.9)	14 (40.0)	10 (28.6)	8 (22.9)	5 (14.3)	2 (5.7)	26 (92.9)
	(n=20)			(n=20)			(n=6)
Non-Heavy Menstrual Bleeding	10 (50)	15 (75)	7 (35)	5 (25)	7 (35)	1 (5.0)	6 (100)

AA=arachidonic acid; C-EPI=collagen-epinephrine; C-ADP=collagen-ADP; DG/PL=dense granules per platelet; EM=electron microscope; ADP= adenosine diphosphate; epi=epinephrine.
P-values exceeded 0.05 for all differences between the 2 groups.
Data are presented as n (%).
Closure times were performed on platelet function analyzer system; Aggregation studies were performed by light transmission aggregometry; Dense granule.
enumeration was performed by whole mount EM of platelet samples.
*Prolonged closure times=2 S.D. above mean RI:C-EPI, 86-143s & C-ADP, 59-106s; Impaired aggregation=2 S.D. below mean RI for each agonist's concentration: ADP:
0.5μM (78.1-99.8%),10μM (82.8-100.3%); AA:0.5mmol/L (75.6-102.8%); Collagen: 2.0μg/mL(75.8-110.0%), Epi:60μM, (76.6-107.5%), 150μM (78.9-107.1%);
Ristocetin:0.5mg/mL (0.0-21.7%), 1.15mg/mL (75.9-107.1%),1.37mg/mL (80.1-104.9%). Normal dense granule numbers per platelet: 4–6.
^§Based on available aggregation studies.

ISSN: 2162-3619