Figure 4

Early passage medulloblastoma cells that survive 10 Gy radiation show elevated expression of HIF2A and ABCA1 . Live non-apoptotic cell populations of 0 Gy (control) and 10 Gy treated (surviving) cells for two newly derived human patient cultures, R001M (A and C) and R026M (B and D), were isolated by FACS. Analysis by qRT-PCR shows HIF2A (A and B) and ABCA1 (C and D) are elevated in surviving cells from both patients. Bars show relative gene expression derived from the mean ΔCt of quadruplicate multiplex assays. The fold-change value for each pair-matched experiment is also indicated (being the relative expression for surviving cells divided by that for control cells, or 2^-ΔΔCt). Error bars (asymmetric on gene expression scale) represent symmetric +/− 1 SD in the Ct space.