Figure 10

ABCA8 , ABCB4 and ABCC8 show distinctive SHH subtype associated expression patterns, relative to other medulloblastoma subgroups and normal cerebellum. Expression data from the three independent validation cohorts (n = 366 patients total) shown as box and whisker plots (quartiles and median indicated by box outline and centreline respectively). Asterisks indicate significance of difference in expression between subgroup of interest and the remaining subgroups (“***” = p-value < 0.001). In addition, comparisons between the subgroup of interest and normal cerebellum (available for the Boston cohort) were significantly different with a p-value of 0.01 or better for all genes shown. [Note that in the Toronto experiments some probe sequence overlap was present between ABCC8 and enzyme component gene SDHC, for the plot indicated with “#”. Analysis of independent SDHC probes in the other analysis sets showed that the SHH subgroup effect seen for the Toronto cohort is likely driven largely or entirely by ABCC8. There were no confounding issues with the probes for ABCC8 used for the Boston or Heidelberg cohorts].