Figure 6From: Opticin, a small leucine-rich proteoglycan, is uniquely expressed and translocated to the nucleus of chronic lymphocytic leukemia cells Western blot of human and bovine OPTC after chemical deglycosylation by TMFS. A) recombinant human OPTC produced in yeast and B) recombinant bovine OPTC. Western blot was performed using a C-terminal anti-OPTC pAb. Lane 1: Untreated recombinant OPTC. Lane 2: Recombinant OPTC treated with TFMS for 30 min. Lanes 3 and 4: Recombinant OPTC treated with TFMS and anisole for 1 and 3 h, respectively. Complete removal of the carbohydrate structures yielded a 39 kDa and a 40–42 kDa band in the human and bovine OPTC, respectively.Back to article page