Skip to main content
Figure 6 | Experimental Hematology & Oncology

Figure 6

From: Opticin, a small leucine-rich proteoglycan, is uniquely expressed and translocated to the nucleus of chronic lymphocytic leukemia cells

Figure 6

Western blot of human and bovine OPTC after chemical deglycosylation by TMFS. A) recombinant human OPTC produced in yeast and B) recombinant bovine OPTC. Western blot was performed using a C-terminal anti-OPTC pAb. Lane 1: Untreated recombinant OPTC. Lane 2: Recombinant OPTC treated with TFMS for 30 min. Lanes 3 and 4: Recombinant OPTC treated with TFMS and anisole for 1 and 3 h, respectively. Complete removal of the carbohydrate structures yielded a 39 kDa and a 40–42 kDa band in the human and bovine OPTC, respectively.

Back to article page