Figure 3

Western blot analyses to validate the specificity of the own- produced anti- OPTC antibody. The precursor OPTC gene was cloned into pcDNA3.1+ vector and transfected into A) mouse TM3 cell line and B) E. coli JM109. Lane 1: Lysate of cells transfected with the vector alone (negative control). Lane 2: Lysate of cells transfected with the OPTC construct. Lane 3: bovine OPTC produced in HEK-293 cells. Our C-terminal pAb detected a 48 kDa human OPTC expressed in TM3 cells, a 37–38 kDa OPTC produced in bacteria JM109 and a 45–50 kDa recombinant bovine OPTC.