Effect of BTZ on CD20 (A) and HLA-DR (B) expression in JY/WT and BTZ-resistant JY cells. Short term incubations of 24 hours and 48 hours were performed with 5nM BTZ for JY/WT, 35nM for JY/35BTZ cells and 100nM BTZ for JY/100BTZ cells. Results depicted are means ± SD of 3–4 separate experiments. * p < 0.05. (C) Western blot analysis of polyubiquitinated cellular proteins prior to and after 24 hours treatment of BTZ; JY/WT (± 10nM BTZ), JY/35 (± 35nM BTZ) and JY/100 (± 100nM BTZ). β-Actin is displayed as a loading control. Western blot image is representative of 3 separate experiments.