Table 2 Identified proteins of differential expression of the MIA PaCa-2 cells in the OT-time dependent group by MALDI-TOF/TOF MS
Spot No. a | Accession number b | Protein name | Ratio of OT-induced differential expression c | |||||
|---|---|---|---|---|---|---|---|---|
Total proteins | Phosphorylated proteins | |||||||
0 | 24 | 48 (h) | 0 | 24 | 48 (h) | |||
1 | P49321e | Nuclear autoantigenic sperm protein | 1.00 | 0.37 | 1.35 | 1.00 | 0.09 | 0.05 |
2 | P14625f | Endoplasmin | 1.00 | 1.17 | 0.26 | -- | -- | -- |
3 | Q14697 e | Neutral alpha-glucosidase AB | 1.00 | 0.41 | 0.89 | -- | -- | -- |
4 | P11142d | Heat shock cognate 71 kDa protein | 1.00 | 0.62 | 0.45 | 1.00 | 0.13 | 0.08 |
5 | P29401 f | Transketolase | 1.00 | 3.23 | 0.77 | -- | -- | -- |
6 | P31948 d | Stress-induced-phosphoprotein 1 | 1.00 | 0.61 | 0.28 | 1.00 | 1.17 | 0.19 |
7 | P30101 d | Protein disulfide-isomerase A3 | 1.00 | 0.30 | 0.06 | -- | -- | -- |
8 | P17987 d | T-complex protein 1 subunit alpha | 1.00 | 0.39 | 0.21 | -- | -- | -- |
9 | P31146 e | Coronin-1A | 1.00 | 0.45 | 0.74 | -- | -- | -- |
10 | P31943 d | Heterogeneous nuclear ribonucleoprotein H | 1.00 | 0.88 | 0.49 | -- | -- | -- |
11 | P07237 e | Protein disulfide-isomerase | 1.00 | 0.37 | 0.84 | -- | -- | -- |
12 | P13489 d | Ribonuclease inhibitor | 1.00 | 0.91 | 0.23 | -- | -- | -- |
13 | P27797 f | Calreticulin | 1.00 | 3.58 | 0.95 | -- | -- | -- |
14 | P06733 f | Alpha-enolase | 1.00 | 2.37 | 0.82 | 1.00 | 0.24 | 0.07 |
15 | Q86VC0 f | 40S ribosomal protein SA | 1.00 | 1.35 | 2.00 | -- | -- | -- |
16 | P52597 e | Heterogeneous nuclear ribonucleoprotein F | 1.00 | 0.31 | 0.73 | -- | -- | -- |
17 | P52907 e | F-actin-capping protein subunit alpha-1 | 1.00 | 0.49 | 0.81 | -- | -- | -- |
18 | O75821 e | Eukaryotic translation initiation factor 3 subunit I | 1.00 | 0.29 | 0.72 | 1.00 | 0.73 | 0.29 |
19 | P47755 e | F-actin-capping protein subunit alpha-2 | 1.00 | 0.43 | 0.87 | 1.00 | 0.20 | 0.03 |
20 | P04083 d | Annexin A1 | 1.00 | 0.87 | 0.48 | -- | -- | -- |
21 | P04406 e | Glyceraldehyde-3-phosphate dehydrogenase | 1.00 | 0.39 | 1.25 | -- | -- | -- |
22 | P04406 e | Glyceraldehyde-3-phosphate dehydrogenase | 1.00 | 0.41 | 1.32 | -- | -- | -- |
23 | P09651 e | Heterogeneous nuclear ribonucleoprotein A1 | 1.00 | 0.17 | 1.39 | -- | -- | -- |
24 | P22626 e | Heterogeneous nuclear ribonucleoproteins A2/B1 | 1.00 | 0.06 | 0.33 | -- | -- | -- |
25 | P52895 e | Aldo-keto reductase family 1 member C2 | 1.00 | 0.48 | 4.02 | -- | -- | -- |
26 | Q9BYE2 d | Serine/threonine-protein kinase 13 | 1.00 | 0.11 | 0.07 | -- | -- | -- |
27 | P08758 d | Annexin A5 | 1.00 | 0.43 | 0.36 | -- | -- | -- |
28 | Q9Y275 d | 14-3-3 protein epsilon | 1.00 | 0.34 | 0.12 | 1.00 | 0.36 | 0.15 |
29 | P31946 d | 14-3-3 protein beta/alpha | 1.00 | 0.39 | 0.22 | 1.00 | 0.31 | 0.74 |
30 | P63104 d | 14-3-3 protein zeta/delta | 1.00 | 0.42 | 0.28 | 1.00 | 0.87 | 0.14 |
31 | P56537 e | Eukaryotic translation initiation factor 6 | 1.00 | 0.39 | 0.47 | 1.00 | 0.76 | 0.41 |
32 | P62258 f | Tumor necrosis factor ligand superfamily member 13B | 1.00 | 2.13 | 0.29 | 1.00 | 0.33 | 0.17 |
33 | Q6ZU15 e | Septin-14 | 1.00 | 0.04 | 0.78 | -- | -- | -- |
34 | Q13162 e | Peroxiredoxin-4 | 1.00 | 0.20 | 0.53 | -- | -- | -- |
35 | P18669 e | Phosphoglycerate mutase 1 | 1.00 | 0.08 | 2.59 | -- | -- | -- |
36 | P63244 e | Guanine nucleotide-binding protein subunit beta-2-like 1 | 1.00 | 0.49 | 0.91 | -- | -- | -- |
37 | P04792 d | Heat shock protein beta-1 | 1.00 | 0.43 | 0.28 | -- | -- | -- |
38 | P30041 d | Peroxiredoxin-6 | 1.00 | 0.89 | 0.37 | -- | -- | -- |
39 | P09211 d | Glutathione S-transferase P | 1.00 | 0.77 | 0.42 | 1.00 | 0.26 | 0.04 |
40 | P16949 e | Stathmin | 1.00 | 0.35 | 0.46 | -- | -- | -- |
41 | P15531 d | Nucleoside diphosphate kinase A | 1.00 | 0.97 | 0.09 | -- | -- | -- |
42 | P32119 e | Peroxiredoxin-2 | 1.00 | 0.37 | 0.49 | -- | -- | -- |
43 | P60660 d | Myosin light polypeptide 6 | 1.00 | 0.88 | 0.23 | -- | -- | -- |
44 | Q8N1F1f | Putative uncharacterized protein NCRNA00188 | 1.00 | 1.15 | 0.50 | -- | -- | -- |
45 | P05109 e | Protein S100-A8 | 1.00 | 0.29 | 0.73 | -- | -- | -- |
46 | P62937 e | Peptidyl-prolyl cis-trans isomerase A | 1.00 | 0.07 | 0.19 | -- | -- | -- |
47 | Q969J3 | Loss of heterozygosity 12 chromosomal region 1 protein | -- | -- | -- | 1.00 | 0.13 | 0.02 |
48 | P63241 | Eukaryotic translation initiation factor 5A-1 | -- | -- | -- | 1.00 | 0.14 | 0.05 |