Inhibition of transketolase by oxythiamine altered dynamics of protein signals in pancreatic cancer cells

Table 1 Identified proteins of differential expression of the MIA PaCa-2 cells in the OT-dose dependent group by MALDI-TOF/TOF MS

Spot No. ^a	Accession number ^b	Protein name	Ratio of OT-induced differential expression ^c
Spot No. ^a	Accession number ^b	Protein name	0	5	50	500 (μM)
1	P14625	Endoplasmin	1	0.27	0.33	0.20
2	P11142	Heat shock cognate 71 kDa protein	1	0.41	025	0.19
3	P11142	Heat shock cognate 71 kDa protein	1	3.13	2.58	2.79
4	P13639	Elongation factor 2	1	0.13	0.09	0.21
5	P08238	Heat shock protein HSP 90-beta	1	0.26	0.18	0.14
6	P07237	Protein disulfide-isomerase	1	0.07	0.22	0.09
7	P13489	Ribonuclease inhibitor	1	0.17	0.24	0.19
8	P22626	Heterogeneous nuclear ribonucleoproteins A2/B1	1	7.51	11.45	10.96
9	P63244	Guanine nucleotide-binding protein subunit beta-2-like 1	1	0.65	0.43	0.38
10	Q15056	Eukaryotic translation initiation factor 4H	1	0.32	0.15	0.27
11	P56537	Eukaryotic translation initiation factor 6	1	0.40	0.24	0.17
12	P09211	Glutathione S-transferase P	1	0.61	0.57	0.26
13	P30086	Phosphatidylethanolamine-binding protein 1	1	0.63	0.29	0.3
14	P62826	GTP-binding nuclear protein Ran	1	0.37	0.42	0.15
15	P60660	Myosin light polypeptide 6	1	0.34	0.49	0.08
16	P51854	Transketolase	1	4.17	3.30	1.85
17	Q6GMP2	Alpha-enolase	1	0.89	2.13	2.29
18	P31946	14-3-3 protein beta/alpha	1	0.76	0.50	0.55

a. The numbers of the identified protein spots are indicated in Figures 1, 2 and 3;
b. Swiss-Prot/TrEMBL primary accession number.
c. The ratio of expression level (intensity on the gel) of each protein spot were calculated based on the control (0 μM OT) group.

ISSN: 2162-3619