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Table 3 In vivo tumorigenicity assay of FACS sorted CD133 high and CD133 dim LC-42 cells at different injected cell doses in NOD/SCID mice

From: In vitro and in vivo properties of CD133 expressing cells from human lung cancer cell lines

Group Cell type Number of the cells injected Number of the mice with tumor/ number of the mice injected Latency
No. injected
1 CD133high 1×103 3/3 6W
2 CD133dim 1×103 3/3 6W
3 CD133high 1×104 3/3 5W
4 CD133dim 1×104 3/3 5W
5 CD133high 1×105 5/5 3W
6 CD133dim 1×105 5/5 3W
7 CD133high 1×106 3/3 2W
8 CD133dim 1×106 3/3 2W
1 Unsorted cells 1×105 3/3 3W
2 Unsorted cells 2×105 3/3 3W
3 Unsorted cells 2×106 3/3 2W
4 Unsorted cells 5×106 6/6 2W
  1. Comparison of xenograft formation in NOD/SCID mice with different numbers of injected CD133high and CD133dim cells. Both FACS isolated CD133high and CD133dim LC-42 cells were 100% tumorigenic in NOD/SCID mice at different injected cell dose even only 1×103 cells were injected. Unsorted LC-42 cells were established as control groups.