In vivo tumorigenicity assay of FACS sorted CD133high
LC-42 cells in NOD/SCID mice. A. Comparison of xenograft formation in NOD/SCID mice with different injected numbers of CD133high and CD133dim cells. Injected cell dose was found to be related with the time of the bulk tumor appearance and tumour size. When 1000 cells were injected, both CD133high and CD133dim LC-42 cells formed tumors at the 5th week, while only 2 weeks was required when 1×106 cells were injected. B. Representative xenografts from the CD133high and CD133dim LC-42 cells derived from the different injected cell doses. No significant difference of growth appeared between CD133high and CD133dim cells.