Figure 5

The correlation between SP cells and CD133 antigen expression in LC-42 cell line. A. One representative experiment of the CD133 antigen expression in the isolated SP and non-SP cells. The percentage of the CD133⁺ cells in LC-42 cell line is 51.27% (c): percentage of CD133⁺ cells was 33.75% in SP (d) and 42.19% in non-SP (e), demonstrating no significant difference of the CD133 antigen expression between SP and non-SP cells. B. One representative experiment of the SP in the isolated CD133^high and CD133^dim cells. A distinct SP was detected in both isolated CD133^high (b-1) and CD133^dim (c-1) LC-42 cells, and no significant difference of the SP between CD133^high and CD133^dim cells. Cells with added verapamil were used as the blocking control (b-2 and c-2).