Figure 4

Ornithine transcarbamylase (OTC) is expressed in both hMSCs and T-ALL cells and BCT-10 treatment could suppress OTC expression significant in T-ALL cells but only modestly on MSCs. (a) Basal expression of OTC in hTertMSCs, hMSCs from 2 healthy donors, CCRF-CEM, Jurkat and MOLT-4. Cells were harvested and respective proteins were extracted from the corresponding cell lines. SDS-PAGE was performed to separate the proteins. OTC and β-actin (internal control) expression were probed by the corresponding antibodies and then visualized by enzyme coupled luminescence (ECL). (b) Expression of OTC in hMSCs after BCT-100 treatment. hTertMSCs or hMSCs from a healthy donor were cultured in different doses of BCT-100 (0 U/ml, 0.5 U/ml, 1 U/ml and 2 U/ml) for 36 hours and protein was extracted from the cell culture. SDS-PAGE followed by Western blotting was performed. The expression of OTC and β-actin (internal control) of hTertMSCs is presented in the figure. (c), (d) and (e) Expression of OTC in CCRF-CEM, Jurkat or MOLT-4 after BCT-100 treatment respectively. CCRF-CEM, Jurkat and MOLT-4 were cultured in different doses of BCT-100 (0 U/ml, 0.5 U/ml, 1 U/ml and 2 U/ml) for 36 hours and protein was extracted. SDS-PAGE followed by Western blotting was performed. The expression of OTC and β-actin (internal control) of CCRF-CEM, Jurkat or MOLT-4 is presented in the figures. The most representative results are presented.