Figure 3

Apoptosis induced by BCT-100-treated T-ALL cells could be rescue by citrulline but not ornithine .(a) The relationship between BCT-100 action and L-arginine metabolism. (b) Apoptosis of BCT-100-treated T-ALL cell lines could not be rescued by ornithine in 2 of the 3 T-ALL cell lines tested. CCRF-CEM, Jurkat or MOLT-4 cell lines were treated with BCT-100 with or without ornithine (doses ranged from 1 mM to 10 mM) for 36 hours. Annexin V / FITC Propidium iodide (AV/PI) assay was performed to test the percentage of apoptosis. Only CEM showed a modest reduction of BCT-100 induced apoptosis after the addition of ornithine. The average apoptotic percentages of the 3 T-ALL cell lines were derived from 3 independent experiments for each T-ALL cell line with error bars showing standard deviation. **: p < 0.01 between BCT-100 only and BCT-100 + different doses of ornithine. ***: p < 0.005 between BCT-100 only and BCT-100 + different doses of ornithine. (c) Apoptosis of BCT-100-treated T-ALL cell lines could be rescued by citrulline. CCRF-CEM, Jurkat or MOLT-4 were treated with BCT-100 with or without 0.1 mM citrulline for 36 hours. Annexin V / FITC Propidium iodide (AV/PI) assay was performed to test the percentage of apoptosis. All 3 T-ALL cells showed a reduction in apoptosis after the addition of citrulline. The average apoptotic percentages of the 3 T-ALL cell lines were obtained after triplicated experiment of the corresponding T-ALL cell line with error bars showing standard deviation. *: p < 0.05 between BCT-100 only and BCT-100 + 0.1 mM citrulline. ***: p < 0.005 between BCT-100 only and BCT-100 + 0.1 mM citrulline.