Figure 2From: BIRC6 (APOLLON) is down-regulated in acute myeloid leukemia and its knockdown attenuates neutrophil differentiation Significant induction of BIRC6 mRNA levels upon neutrophil differentiation of AML cells. (A) BIRC6 qPCR analysis of NB4 APL cells treated with 1 μM ATRA for 4 and 6 days. Raw Ct values were normalized to HMBS and to the untreated control of day 4. Results from four independent experiments in duplicates are shown. (B) BIRC6 qPCR analysis of ATRA-resistant NB4-R2 APL cells treated as in A. (C) CD11b surface expression of NB4 and NB4-R APL cells upon six days of ATRA-induced neutrophil differentiation. CD11b was measured by FACS analysis. (D) Neutrophil differentiation of HL60 AML cells with 1 μM ATRA for 4 and 6 days, respectively. BIRC6 mRNA expression was assessed by qPCR as in A. (E) BIRC6 qPCR analysis of ATRA-resistant HL60-R cells treated as in A. (F) The differentiation levels of HL60 and HL60-R were assessed by CD11b surface expression upon 6 days of ATRA-induced neutrophil differentiation. Mann–Whitney U test *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant.Back to article page