Figure 3
From: JAK2V617F and p53 mutations coexist in erythroleukemia and megakaryoblastic leukemic cell lines
Verification of the presence of JAK2V617F in HEL and SET-2 cells. DNA fragments containing JAK2 exon 14 and full-length JAK2 cDNA were amplified by PCR from genomic DNAs and reverse-transcribed single-strand cDNAs, respectively. DNA sequencing was performed from the reverse direction. Positions of the V617F mutation sites are indicated by arrows. Note that HEL cells carry a homozygous mutation while SET2 cells contain a heterozygous mutation at both genomic DNA and cDNA levels. DNA sequencing was also performed from the forward direction with consistent results (not shown).