Figure 1
From: JAK2V617F and p53 mutations coexist in erythroleukemia and megakaryoblastic leukemic cell lines
Identification of p53 mutation in HEL and SET-2 cells. The entire coding sequence of p53 was amplified by reverse transcription PCR from total RNAs isolated from HEL and SET2 cells together with a normal white blood cell sample for comparison. The positions of the mutation sites are indicated by arrows, and the associated changes in codons and amino acids are shown. Note that the p53 mutations found in HEL and SET2 cells are homozygous. DNA sequencing was also performed from the reverse direction with consistent results (not shown).