Fig. 5

VISTA triggered changes of cytokine secretion during phagocytosis. A. Measurement of IL-1β in supernatants from VISTA-, ECD-, or EV-transduced THP-1 derived macrophages. B-C. IL-1β concentration in the supernatant of VISTA-, ECD-, or EV-transduced THP-1 derived macrophages after 3 h phagocytosis experiments with Daudi (B) and SU-DHL-2 (C) cells. D-E. IL-1β (D) or IL-10 (E) concentration in the supernatant of VISTA-, ECD-, or EV-transduced CD34⁺ CB-derived macrophages after phagocytosis experiment with Daudi cells. F-G. IL-1β (F) and IL-10 (G) concentration in the supernatant of VISTA-, ECD-, or EV-transduced CD34⁺ CB-derived macrophages after phagocytosis experiment with SU-DHL-2 cells. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001 by one-way ANOVA with post hoc intergroup comparisons using Dunnett’s test