Fig. 2

VISTA elicited transcriptional and surface-expression changes of immune checkpoints. (A) Dot plot with percentage of cells in VSIR-negative or VSIR-positive tumor-associated macrophages (TAMs) expressing immune checkpoints and normalized expression levels for these immune checkpoints within the two TAM groups. n.s., not significant. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001 by two-sided Wilcoxon rank-sum test. (B) Expression level of the indicated genes in VISTA or EV transduced THP-1 cells. C-E. Expression level of the indicated genes in M0-like (C), M2-like (D), and M1-like (E) macrophages derived from VISTA or EV transduced CD34⁺ CB cells. n.s., not significant. ^***P < 0.001 by student’s t-test