Fig. 1

ZMIZ2 is overexpressed in HCC and high ZMIZ2 is correlated with poor clinical prognoses. (A) ZMIZ2 mRNA expression levels in 26 common human tumors were analyzed based on data from The Cancer Genome Atlas (TCGA) database. The log (fold change) of each type cancer versus adjacent normal tissue was shown in the right table. (B) The risk ratio of ZMIZ2 in 26 common human tumors was analyzed via a forest plot. (C) Paired Student’s t-test analysis of ZMIZ2 expression in paired samples of TCGA-LIHC cohort. (D) ZMIZ2 mRNA expression levels in HCC were significantly higher than that in normal tissues according to the GSE54236, GSE55092, and GSE144269 datasets. (E, F) mRNA expressions (E) and protein levels (F) of ZMIZ2 were decreased in a panel of HCC cell lines compared with that in immortalized LO2 human hepatocytes. (G, H) The protein expression of ZMIZ2 in HCC tissues and adjacent normal tissues was detected by western blotting (G) and immunohistochemistry (H) analysis. Scale bars: 100 or 20 μm. (I) Kaplan–Meier plots representing the prognostic values of ZMIZ2 in HCC patient from the TCGA and GSE54236 cohorts. Statistical analysis was conducted using the log-rank test. **p < 0.01; ***p < 0.001; **** p < 0.0001