|  | Colorectal Cancer | Gastric Cancer | Breast Cancer | Pancreatic Cancer | Cholangiocarcinoma | Lung Cancer | Hepatocellular Cancer | Bladder Cancer | Esophagus Cancer | Thyroid Cancer | Prostate Cancer | Cervical Cancer | Ovarian Cancer | Head and Neck Cancer | ||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Medium | Advance DMEM/F12 | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ |
HEPES 10 mM | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | |
Glutamax 2 mM | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | |
Antibiotics/antimycotics (1%) | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | √ | |
Cytokines | rhR-Spondin-1 (ng/ml) | 500 | 250 | 1000 | 250 | Â | Â | Â | 500 | Â | Â | 500 | 250 | 125 | 250 | Â | 100 |
rhNoggin (ng/ml) | Â | Â | Â | 100 | 1000 | 100 | Â | 100 | Â | Â | 100 | 25 | 100 | 100 | Â | 40 | |
rmNoggin (ng/ml) | 100 | 100 | 100 | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | |
rhFGF basic (ng/ml) | Â | Â | Â | Â | Â | Â | Â | Â | Â | 12.5 | Â | 20 | 1 | Â | 50 | 5 | |
rhFGF-7 (ng/ml) | Â | Â | Â | 5 | Â | Â | Â | 25 | Â | 25 | Â | Â | Â | 25 | Â | Â | |
rhFGF-10 (ng/ml) | Â | Â | 50 | 20 | 100 | Â | Â | 100 | 100 | 100 | 100 | Â | 10 | 100 | Â | 10 | |
rhIGF (ng/ml) | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | 100 | Â | |
rhEGF (ng/ml) | 50 | Â | Â | 5 | 50 | Â | Â | Â | 50 | 10 | 50 | 20 | 50 | 50 | Â | 50 | |
rmEGF (ng/ml) | Â | 50 | 50 | Â | Â | 50 | 50 | Â | Â | Â | Â | Â | Â | Â | Â | Â | |
rhWnt-3a (ng/ml) | 50 | 50 | 25 | Â | 50 | 25 | Â | Â | Â | Â | 50 | 50 | Â | Â | 20 | Â | |
rhVEGF121 (ng/ml) | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | 10 | Â | Â | Â | Â | |
rhNeuregulin 1 (nM) | Â | Â | Â | 5 | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | |
rhHGF (ng/ml) | Â | Â | Â | Â | Â | Â | Â | Â | 25 | Â | Â | Â | Â | Â | Â | Â | |
Additive | N-2 | 1 | Â | Â | Â | 1 | Â | 1 | Â | 1 | Â | 1 | Â | Â | Â | Â | Â |
 N-21 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |  | 0.5 | 1 | 1 | 1 | 1 | |
Small Molecular Compounds | Wnt-C59 | Â | Â | Â | Â | Â | 100 | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Y-27,632 (µM) |  |  |  | 5 |  |  | 10 | 5 | 10 | 10 |  | 10 | 10 | 10 | 10 |  | |
A83-01 (nM) | 500 | 500 | 500 | 500 | 500 | 500 | 5000 | 500 | 5 | 5000 | 500 | 5000 | 500 | 500 | 500 | Â | |
Dihydrotestosterone (DHT) (nM) | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | 0.1/1 | Â | Â | Â | |
Primocin | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | 1:100v/v | Â | Â | Â | |
Gastrin I (Human) (nM) | 10 | 10 | 10 | Â | 10 | Â | 10 | Â | 10 | Â | Â | Â | Â | Â | 10 | Â | |
Forskolin (µM) |  |  |  |  |  |  | 10 |  | 10 |  |  |  |  | 10 |  | 1 | |
Dexamethasone (nM) | 10 | 10 | Â | Â | Â | Â | Â | Â | 3 | Â | Â | Â | Â | Â | Â | Â | |
SB 202,190 |  |  |  | 500 nM | 10 mM | 10 µM |  |  |  |  |  |  | 10 µM | 1 µM |  |  | |
Prostaglandin E2 (µM) |  |  |  |  |  |  |  | 500 |  |  |  |  |  |  |  | 1 | |
Beta-Estradiol (nM) | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | 100 | Â | Â | |
Wnt Surrogate (nM) | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | 0.5 | Â | Â | |
CHIR 99,021 (µM) |  |  |  |  |  |  |  |  |  |  |  |  |  | 0.3 |  | 3 | |
Nicotinamide (mM) | 10 | Â | Â | 5 | 10 | Â | 10 | 5 | 10 | 10 | 10 | 0.1 | 10 | 2.5 | Â | Â | |
 N-Acetylcysteine (mM) | 1.25 | 1 | 1 | 1.25 | 1 | 1 | 1 | 1.25 | 1.25 | 1.25 |  |  | 1.25 | 1.25 | 1 | 1.25 | |
References | Â | [79] | [82] | [136] | [51] | [50] | [170] | [102] | [171] | [15] | [172] | [149] | [173] | [10] | [174] | [175] | [176] |