Fig. 1

The LN induces increases in the S + G2/M phases of the cell cycle in HSC. Freshly isolated HSC (a) or HSC from the different niches (b NN10, c NN1, d LN) were stained with Hoechst 33342 dye for cell cycle evaluation by flow cytometry (FACSAriaTM II, BD Biosciences). Areas under the histograms were used to determine the percentage of cells in sub-G0, G0/G1, S and G2/M phases of the cell cycle. FlowJo and FCS Express Flow Cytometry Data Analysis Softwares v5.0 (BD Biosciences) were used for data analysis. A representative experiment is shown