Near complete response after single dose of nivolumab in patient with advanced heavily pre-treated KRAS mutant pulmonary adenocarcinoma
© Davar et al. 2015
Received: 16 October 2015
Accepted: 6 December 2015
Published: 14 December 2015
The programmed death 1 (PD-1) receptor is expressed by activated T-cells and engaged by ligands PD-L1 and PD-L2 normally expressed by infiltrating immune cells in response to viral infection. The PD-1/PD-L1 axis is a negative inhibitory pathway that down-regulates T-cells but is also used by tumors to evade anti-tumor immunity. Antibodies targeting PD-1/PD-L1 axis are capable of restoring functional anti-tumor immunity and have demonstrated efficacy in a broad range of tumor types including non-small cell lung cancer in both squamous and adenocarcinoma histologies. Ongoing issues affecting clinical development of these agents include assessment of response, optimal duration of therapy in excellent responders, predictive biomarkers and mechanisms of resistance. In this report, we describe a patient with advanced KRAS mutant heavily pretreated pulmonary adenocarcinoma who developed an excellent response after a single-dose of nivolumab. Pre-treatment tumor was found to have moderate CD3 and PD-L1 positivity by immunohistochemical staining. Evaluation of exceptional responders and non-responders are critical to furthering our understanding of the mechanisms of action (and resistance) to these agents.
KeywordsLung cancer Adenocarcinoma KRAS mutant Nivolumab Programmed death receptor-1 (PD-1)
In the past decade, large scale sequencing efforts unraveled the role of molecular driver events in the etiopathogenesis of non-small cell lung cancer (NSCLC), primarily in adenocarcinoma. Molecular targeted therapies designed to exploit these weaknesses have transformed the management of the minority of patients with advanced adenocarcinoma whose tumors harbor mutations in epidermal growth factor receptor (EGFR) or rearrangements in anaplastic lymphoma kinase (ALK) or c-ros oncogene 1 (ROS1). Unfortunately, little progress has been made in the treatment of patients with the most frequently observed driver oncogene, mutant KRAS. KRAS is mutated in one-third of all malignancies and approximately 25 % of all NSCLC . Further, acquired resistance to the currently targetable driver mutations is all but inevitable [2, 3]. Moreover, the prognosis of patients whose tumors do not harbor these genetic changes or those who progress on these agents continue to be treated with chemotherapy and have a median survival of 10–12 months.
Programmed death 1 (PD-1) receptor is an inhibitory T cell receptor expressed by activated T-cells and engaged by ligands PD-L1 and PD-L2 of the B7-ligand superfamily normally expressed by infiltrating immune cells in response to viral infection. PD-1/PD-L1 axis constitutes a negative regulatory mechanism by which T-cell activation is homeostatically regulated; but is hijacked by tumors to circumvent effective anti-tumor immunity [4–6]. PD-1 blockade has been explored as an immunotherapeutic strategy with resounding success in immunogenic tumors (melanoma, renal cell carcinoma, urothelial carcinoma) and additionally in tumors not formerly thought immunogenic including squamous NSCLC. Regulatory approval in squamous NSCLC was granted in early 2015 on the basis of improved survival compared to docetaxel in a 2nd line phase III study though activity has been reported in non-squamous NSCLC as well . In these (and other studies) patients are typically first evaluated for response at 12 weeks. Herein, we report a patient with advanced KRAS mutant pulmonary adenocarcinoma treated with nivolumab after progressing on multiple therapies who had a remarkable response after just a single dose.
A 77-year-old African-American female heavy current smoker presented with dyspnea in September 2012. Initial computer tomography imaging revealed bilateral upper lobe masses with mediastinal lymphadenopathy and bilateral pulmonary and pleural nodules consistent with malignancy. Biopsy revealed CK7/TTF-1 positive adenocarcinoma. Molecular studies were notable for a KRAS exon 2 (pG12C, c.34G>T) mutation and TP53 (pR283P, c.848G>C) but otherwise negative for 48 other key cancer genes as determined by the Ampliseq Cancer Hotspot Panel v2 including EGFR/BRAF/PIK3CA mutations, ALK/ROS1/KIF5B/RET rearrangements and MET amplification.
She received 10 fractions of radiation therapy (RT) to mediastinum, then carboplatin and pemetrexed for 4 cycles with a partial response that lasted 5 months before progressing in June 2013. Between June 2013 and January 2015, she received docetaxel, an investigational FAK inhibitor, gemcitabine and pemetrexed. In January 2015, she developed non-infectious pericarditis and spinal metastases requiring RT during which systemic therapy was interrupted till May 2015. Restaging scans documented further pulmonary and mediastinal lymph node progression with no evidence of extra-thoracic metastases. She then received nivolumab 3 mg/kg on May 20, 2015. Two weeks after her first dose, she was subsequently admitted for failure to thrive and dysphagia and was found to have a severe esophageal stricture near the GE junction secondary to extrinsic compression. She was subsequently discharged to a skilled nursing facility with plan for hospice. Two weeks after discharge, a dramatic clinical improvement was observed (weight gain, resolution of dysphagia and improved performance status) and the patient was seen in clinic. Her course was subsequently complicated by exacerbation of chronic obstructive pulmonary disease (COPD) requiring admission at which time a restaging CT scan was obtained, then convalescence in a nursing home, which precluded further nivolumab administration despite a remarkable improvement in dysphagia and performance status. Restaging scans showed marked regression in dominant parenchymal lung masses and para-esophagal lymph node commensurate with improving performance status and subjective dysphagia. She has since received 3 further doses of nivolumab with ongoing response on last restaging scans on November 13, 2015.
PD-1/PD-L1 staining is highly contextual and depends on the disease and treatment setting. Evaluation of PD-1/PD-L1 staining in archival samples indicates that staining varies widely in solid tumors being highly expressed in certain tumors (melanoma, sarcoma) but minimally in others (hepatocellular carcinoma) . Within tumors, PD-1/PD-L1 expression appears to be prognostic—being associated with poorer prognoses in multiple tumor types including testicular germ cell tumors and prostate cancer [13, 14]. The observation of PD-L1 upregulation on leukemic cells in patients on treatment with bi-specific antibodies likely represents development of a T-cell-induced immune escape mechanism—and suggests that PD-1/PD-L1 inhibition may be complementary to these therapies in this setting [15, 16]. It is likely that the most accurate biomarkers will incorporate elements that reflect the dynamic and complex nature of the immune system including tumor mutation burden and T-cell infiltrate [17, 18]. Close interrogation of the host, tumor and tumor microenvironment in both extraordinary responders and primary non-responders to PD-1 therapy will be critical to this effort.
Written informed consent for the publication of details, laboratory results and images relating to individual participants was obtained from the participants for publication of this case report and any accompanying images. Copies of the written consents are available for review by the Editor-in-Chief of this journal.
non-small cell lung cancer
epidermal growth factor receptor
anaplastic lymphoma kinase
c-ros oncogene 1
programmed death 1
chronic obstructive pulmonary disease
DD, MAS and TFB conceived of this study report, collected the data, wrote and revised the manuscript. TFB was responsible for treatment decisions. SD reviewed initial and subsequent pathology and performed immunohistochemical stains described in attached figures. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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